| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 31 | Holdemania filiformis | GCCAGCTGCGCAATGAAA | CCAGACAATGATCGACGGC | 59.74 | 58.70 | 171 | 99 |
100.00%
|
50.00%
|
| Primer 32 | Holdemania filiformis | GCCAGCTGCGCAATGAAA | CCAGACAATGATCGACGGCA | 59.74 | 60.46 | 171 | 99 |
100.00%
|
50.00%
|
| Primer 33 | Holdemania filiformis | TGCCAGACGAAACCCACA | TAATCCTGCAGCTGCGCA | 59.09 | 59.73 | 167 | 99 |
100.00%
|
50.00%
|
| Primer 34 | Holdemania filiformis | CTGCCAGACGAAACCCACA | TAATCCTGCAGCTGCGCA | 60.23 | 59.73 | 168 | 99 |
100.00%
|
50.00%
|
| Primer 35 | Holdemania filiformis | ATCCAGGAGAAGCGACTGC | TAACTGCGCTGTCAGGACC | 59.48 | 59.71 | 168 | 99 |
100.00%
|
50.00%
|
| Primer 36 | Holdemania filiformis | TGCGGTCGCTGTTTTCCA | TACCGTCCACGTTCAAGCC | 60.20 | 60.01 | 299 | 97 |
100.00%
|
50.00%
|
| Primer 37 | Holdemania filiformis | TGCGGTCGCTGTTTTCCA | TTGCCGTCGACGACTAAGC | 60.20 | 60.44 | 231 | 97 |
100.00%
|
50.00%
|
| Primer 38 | Holdemania filiformis | ATCGAACCGCTGCATGGT | CGTCGCGACAATCGTTTCC | 59.73 | 59.94 | 228 | 97 |
100.00%
|
50.00%
|
| Primer 39 | Holdemania filiformis | ATCGAACCGCTGCATGGT | CCGTCGCGACAATCGTTTC | 59.73 | 59.94 | 229 | 97 |
100.00%
|
50.00%
|
| Primer 40 | Holdemania filiformis | CCGGAAAAGGTGGTGTGGT | AAAGCGCATGGAAACGGC | 60.15 | 59.74 | 183 | 97 |
100.00%
|
50.00%
|